Summary
Type: SPE
Application #: 305320
Catalog#: 730747 - SPE Cartridges, Chromabond, HR-XAW, 3mL, 60mg, 30/pk
Detection:
Conditions: Column conditioning: 2 mL methanol, then 2 mL water
(Do not let the column run dry!)
Sample aspiration: 2 mL sample solution is passed through the column by vacuum
Washing: 2 mL 2 % formic acid in water, then 2 mL methanol
Drying: with vacuum
Elution: 2 mL methanol + 5 % ammonia
Evaporation: to dryness (mild nitrogen stream, slightly warming)
Reconstitution: in suitable eluent
Authors: SPE department
Source: Macherey-Nagel (Germany 2009)